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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in many tumors, which ends up in chromosome instability, and thus may contribute to tumorigenesis. This protein was located to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a significant function in promoting cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two added splice variants were being claimed but in depth nucleotide sequence data isn't however available.

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Blocking peptides are peptides that bind particularly into the goal antibody and block antibody binding. These peptide normally incorporates the epitope recognized through the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...

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This guide outlines even more possible optimization of mobile and immunoassay parameters to be sure the best possible success are...

Antibodies sure to the blocking peptide no longer bind to your epitope to the focus on protein. By evaluating the staining with the blocked antibody versus the antibody by yourself, you can see which staining is specific.

Concentrate on Data Cyclin E belongs for the extremely conserved cyclin family, whose users are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute towards the temporal coordination of each and every mitotic function. Cyclin E forms a fancy with and capabilities as being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is degraded as cells progress by way of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...

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